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96
ATCC sars cov 2
Customizable one-pot diagnostic system. ( A ) Schematic illustration comparing two design approaches for one-pot reactions. Created in BioRender, Park, H. ( https://BioRender.com/jaoj8es ). (B–D) Fluorescence analysis of one-pot reactions across varying <t>concentrations</t> <t>of</t> <t>SARS-CoV-2</t> RNA using Cas12a complexes formed with ( B ) crRNA1, ( C ) crRNA2, and ( D ) crRNA3. For each condition, two plots are shown: conventional (left), and decoupling based (right) one-pot strategy at 20 min. All experiments were performed in triplicate; error bars represent mean ± standard deviation ( n = 3). P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant).
Sars Cov 2, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
ATCC heat inactivated foetal bovine serum fbs
Customizable one-pot diagnostic system. ( A ) Schematic illustration comparing two design approaches for one-pot reactions. Created in BioRender, Park, H. ( https://BioRender.com/jaoj8es ). (B–D) Fluorescence analysis of one-pot reactions across varying <t>concentrations</t> <t>of</t> <t>SARS-CoV-2</t> RNA using Cas12a complexes formed with ( B ) crRNA1, ( C ) crRNA2, and ( D ) crRNA3. For each condition, two plots are shown: conventional (left), and decoupling based (right) one-pot strategy at 20 min. All experiments were performed in triplicate; error bars represent mean ± standard deviation ( n = 3). P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant).
Heat Inactivated Foetal Bovine Serum Fbs, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Millipore heat shock proteins
Customizable one-pot diagnostic system. ( A ) Schematic illustration comparing two design approaches for one-pot reactions. Created in BioRender, Park, H. ( https://BioRender.com/jaoj8es ). (B–D) Fluorescence analysis of one-pot reactions across varying <t>concentrations</t> <t>of</t> <t>SARS-CoV-2</t> RNA using Cas12a complexes formed with ( B ) crRNA1, ( C ) crRNA2, and ( D ) crRNA3. For each condition, two plots are shown: conventional (left), and decoupling based (right) one-pot strategy at 20 min. All experiments were performed in triplicate; error bars represent mean ± standard deviation ( n = 3). P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant).
Heat Shock Proteins, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
ATCC s ar s cov 2
Customizable one-pot diagnostic system. ( A ) Schematic illustration comparing two design approaches for one-pot reactions. Created in BioRender, Park, H. ( https://BioRender.com/jaoj8es ). (B–D) Fluorescence analysis of one-pot reactions across varying <t>concentrations</t> <t>of</t> <t>SARS-CoV-2</t> RNA using Cas12a complexes formed with ( B ) crRNA1, ( C ) crRNA2, and ( D ) crRNA3. For each condition, two plots are shown: conventional (left), and decoupling based (right) one-pot strategy at 20 min. All experiments were performed in triplicate; error bars represent mean ± standard deviation ( n = 3). P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant).
S Ar S Cov 2, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
JULABO GmbH heat exchangers
Customizable one-pot diagnostic system. ( A ) Schematic illustration comparing two design approaches for one-pot reactions. Created in BioRender, Park, H. ( https://BioRender.com/jaoj8es ). (B–D) Fluorescence analysis of one-pot reactions across varying <t>concentrations</t> <t>of</t> <t>SARS-CoV-2</t> RNA using Cas12a complexes formed with ( B ) crRNA1, ( C ) crRNA2, and ( D ) crRNA3. For each condition, two plots are shown: conventional (left), and decoupling based (right) one-pot strategy at 20 min. All experiments were performed in triplicate; error bars represent mean ± standard deviation ( n = 3). P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant).
Heat Exchangers, supplied by JULABO GmbH, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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97
ATCC heat inactivated fetal bovine serum hi fbs
Customizable one-pot diagnostic system. ( A ) Schematic illustration comparing two design approaches for one-pot reactions. Created in BioRender, Park, H. ( https://BioRender.com/jaoj8es ). (B–D) Fluorescence analysis of one-pot reactions across varying <t>concentrations</t> <t>of</t> <t>SARS-CoV-2</t> RNA using Cas12a complexes formed with ( B ) crRNA1, ( C ) crRNA2, and ( D ) crRNA3. For each condition, two plots are shown: conventional (left), and decoupling based (right) one-pot strategy at 20 min. All experiments were performed in triplicate; error bars represent mean ± standard deviation ( n = 3). P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant).
Heat Inactivated Fetal Bovine Serum Hi Fbs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
InvivoGen heat killed c albicans
Customizable one-pot diagnostic system. ( A ) Schematic illustration comparing two design approaches for one-pot reactions. Created in BioRender, Park, H. ( https://BioRender.com/jaoj8es ). (B–D) Fluorescence analysis of one-pot reactions across varying <t>concentrations</t> <t>of</t> <t>SARS-CoV-2</t> RNA using Cas12a complexes formed with ( B ) crRNA1, ( C ) crRNA2, and ( D ) crRNA3. For each condition, two plots are shown: conventional (left), and decoupling based (right) one-pot strategy at 20 min. All experiments were performed in triplicate; error bars represent mean ± standard deviation ( n = 3). P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant).
Heat Killed C Albicans, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
ATCC sars cov 2 target sequence
Customizable one-pot diagnostic system. ( A ) Schematic illustration comparing two design approaches for one-pot reactions. Created in BioRender, Park, H. ( https://BioRender.com/jaoj8es ). (B–D) Fluorescence analysis of one-pot reactions across varying <t>concentrations</t> <t>of</t> <t>SARS-CoV-2</t> RNA using Cas12a complexes formed with ( B ) crRNA1, ( C ) crRNA2, and ( D ) crRNA3. For each condition, two plots are shown: conventional (left), and decoupling based (right) one-pot strategy at 20 min. All experiments were performed in triplicate; error bars represent mean ± standard deviation ( n = 3). P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant).
Sars Cov 2 Target Sequence, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
InvivoGen heat killed candida albicans
Customizable one-pot diagnostic system. ( A ) Schematic illustration comparing two design approaches for one-pot reactions. Created in BioRender, Park, H. ( https://BioRender.com/jaoj8es ). (B–D) Fluorescence analysis of one-pot reactions across varying <t>concentrations</t> <t>of</t> <t>SARS-CoV-2</t> RNA using Cas12a complexes formed with ( B ) crRNA1, ( C ) crRNA2, and ( D ) crRNA3. For each condition, two plots are shown: conventional (left), and decoupling based (right) one-pot strategy at 20 min. All experiments were performed in triplicate; error bars represent mean ± standard deviation ( n = 3). P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant).
Heat Killed Candida Albicans, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
ATCC heat inactivated sars cov 2 virus
Customizable one-pot diagnostic system. ( A ) Schematic illustration comparing two design approaches for one-pot reactions. Created in BioRender, Park, H. ( https://BioRender.com/jaoj8es ). (B–D) Fluorescence analysis of one-pot reactions across varying <t>concentrations</t> <t>of</t> <t>SARS-CoV-2</t> RNA using Cas12a complexes formed with ( B ) crRNA1, ( C ) crRNA2, and ( D ) crRNA3. For each condition, two plots are shown: conventional (left), and decoupling based (right) one-pot strategy at 20 min. All experiments were performed in triplicate; error bars represent mean ± standard deviation ( n = 3). P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant).
Heat Inactivated Sars Cov 2 Virus, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Customizable one-pot diagnostic system. ( A ) Schematic illustration comparing two design approaches for one-pot reactions. Created in BioRender, Park, H. ( https://BioRender.com/jaoj8es ). (B–D) Fluorescence analysis of one-pot reactions across varying concentrations of SARS-CoV-2 RNA using Cas12a complexes formed with ( B ) crRNA1, ( C ) crRNA2, and ( D ) crRNA3. For each condition, two plots are shown: conventional (left), and decoupling based (right) one-pot strategy at 20 min. All experiments were performed in triplicate; error bars represent mean ± standard deviation ( n = 3). P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant).

Journal: Nucleic Acids Research

Article Title: Functional decoupling of crRNA enables customizable CRISPR diagnostics

doi: 10.1093/nar/gkag189

Figure Lengend Snippet: Customizable one-pot diagnostic system. ( A ) Schematic illustration comparing two design approaches for one-pot reactions. Created in BioRender, Park, H. ( https://BioRender.com/jaoj8es ). (B–D) Fluorescence analysis of one-pot reactions across varying concentrations of SARS-CoV-2 RNA using Cas12a complexes formed with ( B ) crRNA1, ( C ) crRNA2, and ( D ) crRNA3. For each condition, two plots are shown: conventional (left), and decoupling based (right) one-pot strategy at 20 min. All experiments were performed in triplicate; error bars represent mean ± standard deviation ( n = 3). P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant).

Article Snippet: SARS-CoV-2 (cat. #VR-1986HK) and influenza A virus (cat. #VR-1938) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Diagnostic Assay, Fluorescence, Standard Deviation, Two Tailed Test

Clinical feasibility of the CLUE system. ( A ) Clinical application of the CLUE system using 120 nasopharyngeal swab samples: 30 SARS-CoV-2-positive, 30 influenza A-positive, 30 influenza B-positive, 30 negative control specimens. Error bars represent mean ± standard deviation ( n = 3). The dashed line represents the threshold, determined by the maximum Youden’s index. ( B ) Comparison of fluorescence signals between different patient swab samples, P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant). ( C ) ROC curve generated from clinical samples. The AUC was 1.0. ( D ) Confusion matrix of the clinical results. The sensitivity was 100% (95% CIs: 88.7%–100%) and the specificity was 100% (95% CIs: 95.9%–100%). Statistical significance between the test results and clinical status was determined by Fisher’s exact test (two-sided P < .0001; odds ratio = ∞, 95% CIs 514.4 to ∞).

Journal: Nucleic Acids Research

Article Title: Functional decoupling of crRNA enables customizable CRISPR diagnostics

doi: 10.1093/nar/gkag189

Figure Lengend Snippet: Clinical feasibility of the CLUE system. ( A ) Clinical application of the CLUE system using 120 nasopharyngeal swab samples: 30 SARS-CoV-2-positive, 30 influenza A-positive, 30 influenza B-positive, 30 negative control specimens. Error bars represent mean ± standard deviation ( n = 3). The dashed line represents the threshold, determined by the maximum Youden’s index. ( B ) Comparison of fluorescence signals between different patient swab samples, P- values were calculated by unpaired two-tailed Student’s t -test (**** P < .0001, *** P < .001, ** P < .01, * P < .05, ns = not significant). ( C ) ROC curve generated from clinical samples. The AUC was 1.0. ( D ) Confusion matrix of the clinical results. The sensitivity was 100% (95% CIs: 88.7%–100%) and the specificity was 100% (95% CIs: 95.9%–100%). Statistical significance between the test results and clinical status was determined by Fisher’s exact test (two-sided P < .0001; odds ratio = ∞, 95% CIs 514.4 to ∞).

Article Snippet: SARS-CoV-2 (cat. #VR-1986HK) and influenza A virus (cat. #VR-1938) were obtained from the American Type Culture Collection (ATCC, USA).

Techniques: Negative Control, Standard Deviation, Comparison, Fluorescence, Two Tailed Test, Generated